New: foodproof®PCR kits usable on many more instruments
Merck extends product range of real-time PCR assays
Since January 2008, Merck has been distributing the innovative foodproof® real-time PCR (polymerase chain reaction) products from Biotecon Diagnostics GmbH, Germany, designed for use on Roche's LightCycler® instruments 1.x or 2.0. Now, Merck has extended its range of foodproof® products with new real-time PCR kits which can be used on many other instruments such as TaqMan®-based instruments or Roche's LightCycler® 480.
foodproof® real-time PCR products - your benefits:
- High Efficiency
Relative detection limit is 1-10 bacteria / 25 g or ml sample. Due to the high reliability of real-time PCR technology (excluding SYBR Green technology), there is no need for confirmation of your results.
- High Speed
Optimize costs through fast results – in less than 24 hours including pre-enrichment
- Convenience
Kit includes all reagents necessary for detection as well as positive, negative and internal amplification controls for diagnostic quality assurance
- Superior Safety
All foodproof® real-time PCR detection kits contain uracil N-glycosylase to prevent carry-over contaminations from previously generated PCR products in the lab.
Real-time PCR for quantitative results
All foodproof® products are 'real-time' PCR (RT-PCR) kits. RT-PCR, an advancement of the original PCR technique, allows the 'online' monitoring of the reaction process and is thus suitable for the quantitative detection of bacteria, viruses and other organisms. This is a major advantage over 'standard' PCR kits which require laborious and time-consuming post-PCR steps such as gel electrophoresis to analyze the reactions.
Faster results, easier handling
In order to achieve valid PCR results, correct sample preparation is necessary to eliminate substances inhibiting the Taq polymerase enzyme. For all foodproof® RT-PCR kits, quick and easy-to-use sample preparation kits are available. The proven foodproof® short sample preparation kits (foodproof® ShortPrep I or II, or III kits) can be used for most pathogens assays. Using these kits, fewer handling steps are needed and PCR assay time is reduced to only 30 minutes. Thus, overall testing time takes only about 1 to 1.5 hours. To detect the presence of bacteria, an enrichment time of only 18-24 h is required, hence the time to result is shortened by several days compared to standard methods.
High-quality products for the detection of food pathogens
The foodproof® product line extends Merck's unique range of solutions for the rapid automated or manual detection and confirmation of important food pathogens such as Salmonella, Listeria spp., Listeria monocytogenes, E. coli O157, pathogenic Campylobacter, and other pathogens. All these rapid tests fit perfectly into Merck's comprehensive range of high-quality granulated dehydrated culture media.
| The following foodproof® kits are now available for use on TaqMan-based instruments: |
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| foodproof® Brucella Detection System, TqM Probes |
| foodproof® E. coli O157 Detection System, TqM Probes |
| foodproof® Listeria Genus Detection System, TqM Probes |
| foodproof® Listeria monocytogenes Detection System, TqM Probes |
| foodproof® Salmonella Detection System, TqM Probes |
The proven foodproof® GMO Maize quantification kit and the unique foodproof® kit for the simultaneous detection of Enterobacteriaceae and E. sakazakii in a single reaction can be used on both the microplate-based LightCycler® 480 and the carousel-based LightCycler® 1.x, 2.0 instruments.
foodproof® is a trademark of Biotecon Diagnostics; LightCycler® is a trademark of Roche Diagnostics; TaqMan® is a trademark of Roche Diagnostics.
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